Protein Model Discrimination Using Mutational Sensitivity Derived from Deep Sequencing
نویسندگان
چکیده
منابع مشابه
Protein model discrimination using mutational sensitivity derived from deep sequencing.
A major bottleneck in protein structure prediction is the selection of correct models from a pool of decoys. Relative activities of ∼1,200 individual single-site mutants in a saturation library of the bacterial toxin CcdB were estimated by determining their relative populations using deep sequencing. This phenotypic information was used to define an empirical score for each residue (RankScore),...
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word count: 186 32 Number figures/tables: 3/3 33 Number references: 39 34
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ژورنال
عنوان ژورنال: Structure
سال: 2012
ISSN: 0969-2126
DOI: 10.1016/j.str.2011.11.021